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anti cox1 polyclonal antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti cox1 polyclonal antibody
    Anti Cox1 Polyclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cox1 polyclonal antibody/product/Cell Signaling Technology Inc
    Average 94 stars, based on 11 article reviews
    anti cox1 polyclonal antibody - by Bioz Stars, 2026-05
    94/100 stars

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    A939572 inhibits OC differentiation by modulating arachidonic acid metabolism. (A) Integrated transcriptomic and metabolomic analysis. (B) Heatmap of PGD2‐related metabolites. i indicates inhibitor‐treated samples, NC indicates control samples. (C, D) GSEA of arachidonic acid and mineralization‐related metabolites. (E) PGD2 concentrations in cell measured by ELISA in each group ( n = 6). (F, G) Western blots showing HPGDS, <t>COX1</t> and COX2 protein levels in OCs treated with A939572. (H) Western blots showing c‐Fos and NFATc1 protein levels in BMDMs treated with varying concentrations of PGD2. (I) Changes in OC‐related markers following RANKL stimulation at different time points, with or without 0.625 μM PGD2 treatment. (J) RT‐qPCR analysis of OC‐related markers in response to different concentrations of PGD2. (K) TRAP staining and F‐actin immunofluorescence staining of OCs treated with or without PGD2; TRAP staining, Scale bars, 100 μm; F‐actin immunofluorescence staining, Scale bars, 100 μm. (L) Quantification of nuclei numbers of TRAP‐positive multinuclear cells ( n = 3). Data are presented as mean ± SD. NS, not significant ( p > 0.05), * p ≤ 0.05, ** p < 0.01, *** p < 0.001. Source numerical and statistical data are provided.
    Cox1, supplied by Huabio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti cox1 polyclonal antibody  (Cell Signaling Technology Inc)
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    cox1  (Cell Signaling Technology Inc)
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    A939572 inhibits OC differentiation by modulating arachidonic acid metabolism. (A) Integrated transcriptomic and metabolomic analysis. (B) Heatmap of PGD2‐related metabolites. i indicates inhibitor‐treated samples, NC indicates control samples. (C, D) GSEA of arachidonic acid and mineralization‐related metabolites. (E) PGD2 concentrations in cell measured by ELISA in each group ( n = 6). (F, G) Western blots showing HPGDS, <t>COX1</t> and COX2 protein levels in OCs treated with A939572. (H) Western blots showing c‐Fos and NFATc1 protein levels in BMDMs treated with varying concentrations of PGD2. (I) Changes in OC‐related markers following RANKL stimulation at different time points, with or without 0.625 μM PGD2 treatment. (J) RT‐qPCR analysis of OC‐related markers in response to different concentrations of PGD2. (K) TRAP staining and F‐actin immunofluorescence staining of OCs treated with or without PGD2; TRAP staining, Scale bars, 100 μm; F‐actin immunofluorescence staining, Scale bars, 100 μm. (L) Quantification of nuclei numbers of TRAP‐positive multinuclear cells ( n = 3). Data are presented as mean ± SD. NS, not significant ( p > 0.05), * p ≤ 0.05, ** p < 0.01, *** p < 0.001. Source numerical and statistical data are provided.
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    A939572 inhibits OC differentiation by modulating arachidonic acid metabolism. (A) Integrated transcriptomic and metabolomic analysis. (B) Heatmap of PGD2‐related metabolites. i indicates inhibitor‐treated samples, NC indicates control samples. (C, D) GSEA of arachidonic acid and mineralization‐related metabolites. (E) PGD2 concentrations in cell measured by ELISA in each group ( n = 6). (F, G) Western blots showing HPGDS, <t>COX1</t> and COX2 protein levels in OCs treated with A939572. (H) Western blots showing c‐Fos and NFATc1 protein levels in BMDMs treated with varying concentrations of PGD2. (I) Changes in OC‐related markers following RANKL stimulation at different time points, with or without 0.625 μM PGD2 treatment. (J) RT‐qPCR analysis of OC‐related markers in response to different concentrations of PGD2. (K) TRAP staining and F‐actin immunofluorescence staining of OCs treated with or without PGD2; TRAP staining, Scale bars, 100 μm; F‐actin immunofluorescence staining, Scale bars, 100 μm. (L) Quantification of nuclei numbers of TRAP‐positive multinuclear cells ( n = 3). Data are presented as mean ± SD. NS, not significant ( p > 0.05), * p ≤ 0.05, ** p < 0.01, *** p < 0.001. Source numerical and statistical data are provided.
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    d immitis cox1 amplicons  (Macrogen)
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    A939572 inhibits OC differentiation by modulating arachidonic acid metabolism. (A) Integrated transcriptomic and metabolomic analysis. (B) Heatmap of PGD2‐related metabolites. i indicates inhibitor‐treated samples, NC indicates control samples. (C, D) GSEA of arachidonic acid and mineralization‐related metabolites. (E) PGD2 concentrations in cell measured by ELISA in each group ( n = 6). (F, G) Western blots showing HPGDS, <t>COX1</t> and COX2 protein levels in OCs treated with A939572. (H) Western blots showing c‐Fos and NFATc1 protein levels in BMDMs treated with varying concentrations of PGD2. (I) Changes in OC‐related markers following RANKL stimulation at different time points, with or without 0.625 μM PGD2 treatment. (J) RT‐qPCR analysis of OC‐related markers in response to different concentrations of PGD2. (K) TRAP staining and F‐actin immunofluorescence staining of OCs treated with or without PGD2; TRAP staining, Scale bars, 100 μm; F‐actin immunofluorescence staining, Scale bars, 100 μm. (L) Quantification of nuclei numbers of TRAP‐positive multinuclear cells ( n = 3). Data are presented as mean ± SD. NS, not significant ( p > 0.05), * p ≤ 0.05, ** p < 0.01, *** p < 0.001. Source numerical and statistical data are provided.
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    cox1 sequences  (Hasegawa Co Ltd)
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    Hasegawa Co Ltd cox1 sequences
    A939572 inhibits OC differentiation by modulating arachidonic acid metabolism. (A) Integrated transcriptomic and metabolomic analysis. (B) Heatmap of PGD2‐related metabolites. i indicates inhibitor‐treated samples, NC indicates control samples. (C, D) GSEA of arachidonic acid and mineralization‐related metabolites. (E) PGD2 concentrations in cell measured by ELISA in each group ( n = 6). (F, G) Western blots showing HPGDS, <t>COX1</t> and COX2 protein levels in OCs treated with A939572. (H) Western blots showing c‐Fos and NFATc1 protein levels in BMDMs treated with varying concentrations of PGD2. (I) Changes in OC‐related markers following RANKL stimulation at different time points, with or without 0.625 μM PGD2 treatment. (J) RT‐qPCR analysis of OC‐related markers in response to different concentrations of PGD2. (K) TRAP staining and F‐actin immunofluorescence staining of OCs treated with or without PGD2; TRAP staining, Scale bars, 100 μm; F‐actin immunofluorescence staining, Scale bars, 100 μm. (L) Quantification of nuclei numbers of TRAP‐positive multinuclear cells ( n = 3). Data are presented as mean ± SD. NS, not significant ( p > 0.05), * p ≤ 0.05, ** p < 0.01, *** p < 0.001. Source numerical and statistical data are provided.
    Cox1 Sequences, supplied by Hasegawa Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    A939572 inhibits OC differentiation by modulating arachidonic acid metabolism. (A) Integrated transcriptomic and metabolomic analysis. (B) Heatmap of PGD2‐related metabolites. i indicates inhibitor‐treated samples, NC indicates control samples. (C, D) GSEA of arachidonic acid and mineralization‐related metabolites. (E) PGD2 concentrations in cell measured by ELISA in each group ( n = 6). (F, G) Western blots showing HPGDS, COX1 and COX2 protein levels in OCs treated with A939572. (H) Western blots showing c‐Fos and NFATc1 protein levels in BMDMs treated with varying concentrations of PGD2. (I) Changes in OC‐related markers following RANKL stimulation at different time points, with or without 0.625 μM PGD2 treatment. (J) RT‐qPCR analysis of OC‐related markers in response to different concentrations of PGD2. (K) TRAP staining and F‐actin immunofluorescence staining of OCs treated with or without PGD2; TRAP staining, Scale bars, 100 μm; F‐actin immunofluorescence staining, Scale bars, 100 μm. (L) Quantification of nuclei numbers of TRAP‐positive multinuclear cells ( n = 3). Data are presented as mean ± SD. NS, not significant ( p > 0.05), * p ≤ 0.05, ** p < 0.01, *** p < 0.001. Source numerical and statistical data are provided.

    Journal: The FASEB Journal

    Article Title: SCD1 Regulates Osteoclast Differentiation by Modulating Arachidonic Acid Metabolism and Mitochondrial Homeostasis

    doi: 10.1096/fj.202600672R

    Figure Lengend Snippet: A939572 inhibits OC differentiation by modulating arachidonic acid metabolism. (A) Integrated transcriptomic and metabolomic analysis. (B) Heatmap of PGD2‐related metabolites. i indicates inhibitor‐treated samples, NC indicates control samples. (C, D) GSEA of arachidonic acid and mineralization‐related metabolites. (E) PGD2 concentrations in cell measured by ELISA in each group ( n = 6). (F, G) Western blots showing HPGDS, COX1 and COX2 protein levels in OCs treated with A939572. (H) Western blots showing c‐Fos and NFATc1 protein levels in BMDMs treated with varying concentrations of PGD2. (I) Changes in OC‐related markers following RANKL stimulation at different time points, with or without 0.625 μM PGD2 treatment. (J) RT‐qPCR analysis of OC‐related markers in response to different concentrations of PGD2. (K) TRAP staining and F‐actin immunofluorescence staining of OCs treated with or without PGD2; TRAP staining, Scale bars, 100 μm; F‐actin immunofluorescence staining, Scale bars, 100 μm. (L) Quantification of nuclei numbers of TRAP‐positive multinuclear cells ( n = 3). Data are presented as mean ± SD. NS, not significant ( p > 0.05), * p ≤ 0.05, ** p < 0.01, *** p < 0.001. Source numerical and statistical data are provided.

    Article Snippet: Antibodies against the following targets were used: COX1 (ET1610‐98, HUABIO), COX2 (ET1610‐23, HUABIO), HPGDS (ER60724, HUABIO), TOM20 (ET1609‐25, HUABIO), Tartrate Resistant Acid Phosphatase (TRAP; ab191406, Abcam), SCD1 (ab236868, Abcam), SP7 (ab209484, Abcam), Runx2 (ab133261, Abcam), p65 (8242, Cell Signaling Technology), phospho‐p65 (p‐p65; 3033, Cell Signaling Technology), p38 (9212, Cell Signaling Technology), phospho‐p38 (p‐p38; 4511, Cell Signaling Technology), JNK (9252, Cell Signaling Technology), phospho‐JNK (p‐JNK; 9255, Cell Signaling Technology), Erk (5013, Cell Signaling Technology), phospho‐Erk (p‐Erk; 4370, Cell Signaling Technology), Flag (14793S, Cell Signaling Technology), c‐Fos (31 254, Cell Signaling Technology), NDUFS4 (15849‐1‐APM, Proteintech), β‐actin (66009‐1‐Ig, Proteintech), NFATc1 (sc‐7294, Santa Cruz Biotechnology).

    Techniques: Metabolomic, Control, Enzyme-linked Immunosorbent Assay, Western Blot, Quantitative RT-PCR, Staining, Immunofluorescence